Abstract

BORROTO, Janetsy et al. Antimicrobial action and toxicity against Artemia salina of the dichlormethane extract from Morinda royoc L. roots. Rev Cubana Plant Med [online]. 2011, vol.16, n.1, pp. 34-42. ISSN 1028-4796.

Introduction: plants are a source of natural diversity because of the great variety of compounds that they synthesize. Anthraquinones in particular are an important group of secondary metabolites characterized by their antimicrobial and antioxidant action. Objectives: to evaluate the antimicrobial action of dichloromethane extract from Morinda royoc L. roots as well as its toxicity against Artemia salina. Methods: the antimicrobial action was determined by using the brooth microdilution in 96-well plate. The extract action against 7 Candida spp clinical isolates and against bacteria such as methicillin-resistant Staphylococcus aureus, Staphylococcus aureus ATCC 12598, Enterococus faecales, Escherichia coli, Acinetobacter baumanii, Pseudomonas aeruginosa and Klebsiella pneumoniae was evaluated. The extract toxicity was measured using brine shrimp (Artemia salina) lethality test. Results: the crude extract proved to be active against all the tested Candida species. The lowest minimal inhibitory concentration was 1.95 µg/mL. The extract showed strong inhibitory action against S. aureus, E. faecales, and E. coli. The lowest minimal inhibitory concentration was 31.25 µg/mL. The extract presented moderate toxicity against A. salina. Conclusions: the results showed the potentialities of dichloromethane extract from M. royoc L. roots for the treatment of bacterial and fungal infections.

Keywords : Morinda royoc L; anthraquinones; antimicrobial; Artemia salina.

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