Abstract

SANCHEZ CALERO, Janet; FAURE GARCIA, Roberto  and  MITJAVILA CORS, Maria Teresa. Effect of Rhizophora mangle L. on the superoxide anion production in RAW 264.7 murine macrophages. Rev Cubana Plant Med [online]. 2012, vol.17, n.3, pp. 223-232. ISSN 1028-4796.

Introduction: the aqueous extract of Rhizophora mangle L. (red mangrove) bark has some pharmacological properties, that is, the treatment of bovine mastitis, wound healing, uterine infections and stomach ulcers, due to its antiseptic, healing, antiinflammatory and antioxidant properties. However, the studies of antioxidant activity at all complexity levels have not been completed in order to elucidate the mechanisms of action involved in this pharmacological effect. Objective: to determine the effect of Rhizophora mangle aqueous extract and its polyphenolic fraction on superoxide anion production in a cellular line of RAW 264.7 murine macrophages, stimulated with phorbol 12-myristate 13-acetate or lipopolysaccharide. Methods: the evaluation of the antioxidant activity of Rhizophora mangle extract and its polyphenolic fraction on superoxide anion production in RAW 264.7 macrophages activated with lipopolysaccharide or phorbol 12-myristate 13-acetate was made using the C ferricytochrome reduction method. Results: Rhizophora mangle extract and its polyphenolic fraction inhibit the superoxide anion production in RAW 264.7 macrophages activated with both types of agents depending on tannin concentration. The comparison of the regression lines showed significant differences (p< 0.05), resulting this effect higher in the fraction for both types of agents, and the presence of lipopolysaccharide for both samples. Conclusions: Rhizophora mangle aqueous extract showed antioxidant activity at cellular level, evidenced by reduction of oxidative stress in macrophages, through the inhibition of superoxide anion production. At the same time, it was shown that polyphenolic compounds, present in the extract, were the main responsible for the antioxidant effects observed in this study.

Keywords : antioxidant activity; superoxide anion; RAW 264.7 macrophages; Rhizophora mangle L; polyphenolic compounds.

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