Abstract

INFANTE TAVIO, Nadia Inés; ESCALONA VELOZ, Rafael; SIERRA CALZADO, Lillian  and  PALACIOS ROQUE, Guillermo. Usefulness of the digital holographic microscopy for the histomorphometric study of human red blood cells. MEDISAN [online]. 2016, vol.20, n.11, pp.2345-2353. ISSN 1029-3019.

A descriptive and cross-sectional investigation to determine the usefulness of the digital holographic microscopy in the histomorphometric study of human red blood cells, by means of the comparative analysis with the optic microscopy, was carried out in the Digital Holography Department of Oriente University in Santiago de Cuba, from April, 2014 to the same month in 2015. To such an effect, red blood cells samples diluted in the Hepes and Hepa shock-absorbing solutions were selected, Holodig program and Matlab® system were used for the measurement in the holographic technique, and in the statistical processing the Student t test was applied for the comparison of means between both microscopic techniques. It was concluded that the digital holographic microscopy allowed to observe the limits and contours of the studied red blood cells and the minimum variations in their form, besides carrying out morphometric studies which results showed similarity to those obtained with the optic microscopy, and of other parameters, as volume

Keywords : histomorphometric study; human red blood cells; digital holographic microscopy; optic microscopy; shock-absorbing solutions.

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