Abstract

CARRERAS SOLIS, Bertha  and  RODRIGUEZ BATISTA, Dayamí. Evaluation of Bacillus thuringiensis Strains for the control of Drosophila melanogaster Maengi. Fitosanidad [online]. 2009, vol.13, n.2, pp. 83-88. ISSN 1818-1686.

The use of Bacillus thuringiensis as biolarvicide is a viable alternative for insect control so the number of known Bacillus thuringiensis strains active on diptera is growing. The characterization of strains of this bacterium isolated from different regions of Cuba is presented. Characterization was based on their insecticidal activity against Drosophila melanogaster, electron microscopy observation of the spores and crystal mixtures and sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) of spore-crystal suspensions. Strains LBT-63 and LBT-87 displayed similarities with the B. thuringiensis subs. israelensis used in this study with regard profile protein and morphology of crystal inclusions. These strains had major proteins of 130, 70 and 27 kDa and amorphous crystal; however, the strain LBT-87 only showed insecticidal activity against D.melanogaster but lower than B. thuringiensis subs. israelensis control

Keywords : Bacillus thuringiensis; Drosophila melanogaster; profile protein; crystal; toxicity.

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