Abstract

HIDALGO GONGORA, Elsa I  and  PEREZ VICENTE, Luis. Morphologic, cultural and biologic differentiation of guignardia citricarpa and guignardia mangiferae in citric fruits of Cuba. Fitosanidad [online]. 2010, vol.14, n.3, pp. 141-152. ISSN 1818-1686.

Thirty five single spore isolates of Guignardia citricarpa (Gm, Phyllosticta citricarpa) from black spot symptoms and four from G. mangiferae (Gm; Phyllosticta capitalensis) associated to black dots (non pathogenic) were obtained in the rind of citrus fruits. The growth and morphology of the colonies, teleomorph and anamorph structures morphology and dimensions in both fungi were studied in PDA, oatmeal agar (AA) and V8 juice agar (V8). A principal components and cluster statistical analysis were performed to determine the characteristics that contribute the most to species differentiation. It were concluded that both species are concurrent in citrus fruits in Cuba and that the most important characteristics to differentiate them were growth in PDA, the perithecia development in vitro, the minimal size of the conidiostrome and the production and release of a yellow pigment in V8: Gc is slow growing in PDA, develop picnidia abundantly in media, the conidiostrome is frequently smaller than 145 µm, do not develop perithecia in PDA (heterotallic) and release a yellow pigment in V8. Gm es fast growing in PDA, frequently has a conidiostrome higher than 145 µm, develops perithecia in single spore cultures (homotallic) and do not produce and release a yellow pigment in V8. Those characteristic allow the differentiation of both species in citrus plants.

Keywords : Citrus spp; Guignardia; Phyllosticta; fungic morphology; diagnostic.

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