Abstract

SOSA LOPEZ, Acenet I.; PAZOS ALVAREZ-RIVERA, Victoria; TORRES CAMPOS, Dania  and  CASADESUS ROMERO, Luis. Identification and Characterization of Six Isolates from Genera Bacillus with Antagonistic Capacity against Rhizoctonia solani Künh and Sclerotium rolfsii Sacc. Control. Fitosanidad [online]. 2011, vol.15, n.1, pp.39-44. ISSN 1562-3009.

Microorganism identification is realized by several methods, currently. Classical methods utilize phenotypic, morphologic and physiological characters as differentiation criteria. Commercial miniaturized kits as API 50CHB for Bacillus, helps to characterize physiology of this kind of bacteria in an easy and fast way; furthermore they are useful for identification until species level due to their high precision. Observation of morphology and sporulation, the answer to Gram tinction and some biochemical tests permit, in the case of Bacillus spp., put them within they genera. The objective of this investigation was to characterize morphologic, biochemical and physiologically six isolated of genera Bacillusfrom soil and rhizosphere with antagonistic capability in front of Rhizoctonia solani and Sclerotium rolfsii(Sr.), with the utilization of the API 50 CHB. Three of the isolated proved to belong to species Bacillus subtilis and another one to B. megaterium with over than 95% of reliability, also one isolated of B. licheniformis and another one of B. circulans, both with 82.7%.

Keywords : Bacillus; identification; antagonism; Rhizoctonia solani; Sclerotium rolfsii.

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