Abstract

SOSA LOPEZ, Acenet I et al. Evaluation of Filter Paper Disks Preserving Method in Two Bacillus subtilis Cohn. Strains by Antagonistic Activity on Rhizoctonia solani Kühn. Fitosanidad [online]. 2011, vol.15, n.1, pp.45-50. ISSN 1562-3009.

Antagonistic activity of two Bacillus subtilis strains preserved in filter paper disks at 4°C for a period of three years was evaluated. Strains Bs-21 and Bs-42, isolated from Cuban soil and antagonists to Rhizoctonia solani Rs-10 were choiced. Five colonies from Bs-42 and one from Bs-21, randomly selected were growth in nutrient agar. The antagonism was compared by dual culture method in PDA after 96 h at 30°C. Viability of both strains was 108 CFU/mL. Selected colonies kept purity, morphological characteristics and positive response to Gram stain compared with B. subtilis ATCC 6633. All selected colonies inhibited mycelial growth of R. solani Rs-10, but not the reference strain. Strain Bs-21 had the lower inhibition (78%) while Bs-421 and Bs-422 had the higher percent (98%). Variance analysis showed significant differences in the percentage of inhibition exhibited by tested colonies.

Keywords : Bacillus subtilis; biological conservation; antagonism; Rhizoctonia solani.

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