Resumo

HERRERA PALOMA, Myriam; DAZA FIGUEREDO, Jesús  e  MORENO YERAS, Alfredo. Optic- digital analysis of objective-carry-sample interphase using images obtained with confocal microscope. EAC [online]. 2013, vol.34, n.3, pp. 1-12. ISSN 1815-5928.

The optic specifications of microscopes are significant at the moment to obtain images to will be analyze and process. The interphase, from the objective lens until the carry-sample, is anelement that integrates some optic specifications in the moment to obtain aquality image. Images of different frames using carry-samples of glass and of polymeric membranesare obtained. It is evaluated and characterized the illumination to exit of carry-sample applying image processing algorithms and using some describers of the gray levels distributionand their contrast. Of the results of study the characterization of carry-samples settles down as norm, with the aim of diminishing the error factor when processing a digital image in the analysis of a biological sample.

Palavras-chave : interphase; optic-digital processing; confocal microscope.

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