SciELO - Scientific Electronic Library Online

vol.49 issue3Comparison between two techniques for estimating voluntary intake of female buffaloes under grazing conditions. Technical noteProfile of fatty acids of Longissimus dorsi muscle and productive indicators of sheeps, supplemented with pods of Acacia cochliacantha, in grasslands native to dry tropics author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Cuban Journal of Agricultural Science

Print version ISSN 0864-0408On-line version ISSN 2079-3480


GALLEGO, C et al. Pregnancy rate obtained with four hormonal protocols for fixed-time artificial insemination in nulliparous water buffaloes (Bubalus bubalis). Cuban J. Agric. Sci. [online]. 2015, vol.49, n.3, pp.321-327. ISSN 0864-0408.

A total of 120 nulliparous water buffaloes were used to evaluate the effect of different hormonal protocols with fixed-time artificial insemination on the pregnancy rate. Protocol 1 received 2.0 mg of estradiol benzoate and a progesterone intravaginal device for nine days. After the device removal, 0.5 mg of Prostaglandin F2α, 500 UI of equine chorionic gonadotropin and 0.5 mg of estradiol cypionate were applied. Protocol 2 was similar to 1, but without the chorionic gonadotrophin. In protocol 3, two doses of 0.5 mg of Prostaglandin F2α were injected, with an interval of 11d, and 0.5 mg of estradiol cypionate was added to the last dose. Protocol 4 was similar to 3, but, after 48h of the last Prostaglandin F2α, 0.01 mg of Buserelin Acetate was injected. Time-fixed artificial inseminations were performed between 56 and 58 h (protocols 1, 2 and 3) and between 20 and 24 h (protocol 4) after the last hormonal application. Pregnancy rates in protocol 1 (60.8%), 2 (50. 3 %) and 4 (57.3 %) were superior (P<0.05) to those of protocol 3 (16.0 %). The equine chorionic gonadotrophin had no influence on the increase or decrease of fertility. In the protocol with double doses of Prostaglandin F2α and estradiol cypionate, fertility decreased, but increased when the estradiol was substituted by Buserelin Acetate. It can be concluded that protocols 1, 2 and 3 synchronize the estrum and ovulation with good pregnancy rates, and it is possible to apply fixed-time artificial insemination for increasing birth rate in herds and achieving economic benefits

Keywords : hormonal protocols; fixed-time artificial insemination; nulliparous buffaloes.

        · abstract in Spanish     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License