SciELO - Scientific Electronic Library Online

vol.50 issue1Ruminal degradability and fecal digestibility of hydroponic fresh forage of maize (Zea mays) in domestic goats in Santa Elena, EcuadorBiological effect of tannins from four tropical tree species on in vitro ruminal fermentation indicators author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand




  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO


Cuban Journal of Agricultural Science

Print version ISSN 0864-0408On-line version ISSN 2079-3480


GALINDO, Juana et al. Effect of a raw saponin extract on ruminal microbial population and in vitro methane production with star grass (Cynodon nlemfuensis) substrate. Cuban J. Agric. Sci. [online]. 2016, vol.50, n.1, pp.77-87. ISSN 0864-0408.

An experiment was conducted under in vitro conditions for determining the effect of a raw saponin extract on the ruminal microbial population and in vitro methane production with star grass (Cynodon nlemfuensis) substrate. Treatments were designed according to the quantity of saponin extract: 1) control, without saponins, 2) 0.6 %, 3) 1.2 % and 4) 1.8 % of the DM of raw saponin extract.  The basal diet was star grass (C. nlemfuensis). The saponin extract was obtained from Sapindus saponaria fruit and its saponin content was of 139.5 mg, equivalent of diogenin.mL-1. There was a reduction in protozoa population, regardless the saponin level.  Its effect on the main cellulolytic bacteria, determined by PCR-RT, showed that the amount of Fibrobacter succinogenes was not modified while the values of Ruminococcus albus were 25.92; 26.72; 25.2 and 22.35 CT for the levels 0; 0.6; 1.2 and 1.8 %, respectively. The acetic acid concentration was not modified by the saponins; the propionic was reduced with 1.2 % inclusion.  The concentration of valeric acid was 0.68; 0.62; 0.52 and 0.49 mmol.L-1 for 0, 0.6; 1.2 and 1.8 % of saponin extract, respectively. Saponins increased methanogenic representation and methane production.  It is concluded that the saponin extract modulates the fermentative process on reducing protozoa, does not modify the presence of F. succinogenes and decreases that of R. albus, probably due to the fact that both utilize the same resource, space and carbon source in the rumen.  The quantity of methanogens was higher with 1.2 and 1.8 % coinciding with the highest methane production

Keywords : Fibrobacter succinogenes; Ruminococcus albus; star grass; methanogens.

        · abstract in Spanish     · text in English     · English ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License