Introduction:

Eco-toxicological tests performance is among current legislation requirements for biodegradation processes of pollutants improvement.

Objective:

To apply acute toxicity bioassays for treated soils contaminated with diesel eco-toxicological evaluation.

Materials and methods:

Organization for Economic Cooperation and Development (OECD)guide 207 Procedures were applied in which lettuce (Latuca Sativa) seeds and earthworm (Ensenia Andrei) are used as bioindicators; at test concentrations (1, 5, 25, 50 and 100%). matrices toxic effect was measured calculating mean lethal concentration (LC50).

Results and Discussion:

Toxicity is obtained in soils treated by bioremediation with microorganism’s addition (bio augmentation) 1 and 2 in the experimentation carried out with lettuce seeds. Soils treated by bioremediation with an increase in organic conditioners (improved) 1 and 2 and chemical - biological stabilization for four execution variants are not toxic. Evaluation carried out with earthworms showed toxicity in soils treated by improved bioremediation and chemical-biological stabilization with agromena. A similar result was obtained with the soil treated by bio augmentation 2. Non-toxicity of the soils treated by chemical-biological stabilization with an improved product and bio augmentation 1 was demonstrated.

Conclusions:

The toxic nature of the soils treated by bio augmentation No. 1 and 2, improved bioremediation No. 1 and 2 and chemical-biological stabilization No. 2 and 4, constitute a risk for ecosystem.

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