Abstract

SILVA PUPO, Juan José; PEREZ ALARCON, Rosana; FONSECA CARRASCO, Yanexi  and  RODRIGUEZ RODRIGUEZ, Sergio. In vitro propagation of Salix babylonica L. from nodal segments. Rev cubana ciencias forestales [online]. 2020, vol.8, n.3, pp.410-424.  Epub Sep 02, 2020. ISSN 2310-3469.

The objective of this work was to establish a route for the in vitro micropropagation of Salix babylonica. In the disinfection experiment, three treatments were used with 1% sodium hypochlorite, 0.1 % mercury bichloride and the third a double disinfection. with both disinfecting agents. With this last treatment, 71 % of disinfected nodal segments were obtained. 6-BAP 2 mg L-1 alone and in combination with gibberellic acid (5 mg L-1), AIA (1 mg L-1) and ANA (1 mg L-1) were used in the in vitro establishment experiment. The best response was achieved with the use of BAP only with 31,6 % of establishment and an average length of the nodal segments of 6,47 mm. For the multiplication experiment, a comparison was made of the Palomos-Ríos, Chung and Carrasco and MMULT-1 culture media. The multiplication of the nodal segments was higher in the Palomo-Ríos culture medium with 100 % sprouting and a number of 6,42 nodal segments. In the rooting stage, different concentrations of AIB (0.05 and 0.25 mg L-1) and a treatment without growth regulator were used. The highest rooting percentage was obtained in the basal culture medium without AIB with a 91,6 % response.

Keywords : Salix babylonica; Micropropagation; In vitro culture; Growth regulators..

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