Abstract

VILLAVICENCIO MONTOYA, Jonathan et al. Use of native cellulolytic enzymes from ecuador, in the enzymatic hydrolysis for the ethanol production. cen. az. [online]. 2018, vol.45, n.3, pp. 91-100. ISSN 2223-4861.

This study shows the use of cellulolytic enzymes Bacillus sp. in enzymatic hydrolysis process and their use in the production of second-generation ethanol. For doing this, The Plackett-Burman method was used to discard variables that do not influence the enzymatic hydrolysis process, and subsequently to adjust the model using the factorial optimization design Box-Hunter 2^5-2. Once the conditions of the experiments in the enzymatic hydrolysis process were optimized, the best testwas selected considering the highest glucose yield per 100g bagasse. The fermentative stage was also analyzed, resulting in a possibility of producing 1 liter of second-generation ethanol per 12.83kg bagasse and 77.9 liters per ton of bagasse.

Keywords : Enzyme cocktail; enzyme production; second-generation ethanol..

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