Abstract

BERAN, Milos; EIGNER HENKE, K; SRANK, J  and  MELICHAR, F. Standard test of labelling for efficiency for quality control of no carrier added 90YCL3. Nucleus [online]. 2007, n.42, pp. 62-65. ISSN 0864-084X.

ABSTRACT Particle emitting radionuclides (e.g. b--emitters 90Y and 177Lu, b-emitter 149Tb, Auger electron emitter 165Er or positron emitter 86Y) are more and more frequently used in research and clinical practice for imaging and radionuclide targeted therapy in nuclear medicine. These radiometals, altogether threevalent lanthanides or actinides with high specific radioactivity, coupled to biomolecule carriers (peptides or monoclonal antibodies) through chelating link (e.g. DTPA or DOTA) bind to specific antigens and/or receptors of diseased tissues, which enables the imaging (positron emitters) or destruction (b--, a-, and Auger electron emitter) of the diseased tissue releasing the antigens or carrying the receptors. The radionuclide precursor 90YCl3 (solution of hard b--emitter 90Y in diluted HCl) with high purity and specific activity is already commercially produced and succesfully used in nuclear medicine, e.g. for radioimmunotherapy (RIT) of Lymphoma. Specification and purity of our product obtained using extraction 90Sr/90Y generator (using technology of centrifuge extractors with di-2-ethylhexylphosphoric acid, D2EHPA) is examined and compared to other similar products in this contribution. A standard method for determination of labelling efficiency of the 90YCl3 precursor based on its reaction with DOTA-Tyr3-Octreotide (DOTA-TOC) and ITLC-SG chromatographic separation is described and proposed for the quality control.

Keywords : fluorine 18; fluorodeoxyglucose; liquid column chromatography; positron computed tomography; quality control; radiopharmaceuticals; toxicity; autoradiolysis.

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